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KMID : 0364820180540040384
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Korean Journal of Microbiology
2018 Volume.54 No. 4 p.384 ~ p.397
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Characteristic study and optimization of culture conditions for Bacillus amyloliquefaciens SRCM 100731 as probiotic resource for companion animal
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Ryu Myeong-Seon
Yang Hee-Jong
Jeong Su-Ji
Seo Ji-Won
Ha Gwang-Su
Jeong Seong-Yeop
Jeong Do-Youn
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Abstract
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The aim of this study is to screen the strains of Bacillus spp. possessing safety, probiotic activity, and so on, which can beutilized as probiotic resource for using the feed and supplementfood of companion animal. About 300 isolates were isolatedfrom traditional Korean sauces, four isolates that did not haveor produce the six kinds of B. cereus type vomiting and diarrheatoxin genes, β-hemolytic, and three kinds of carcinogenicenzymes were selected. Antibiotic gene retention, cell surfacehydrophobicity, antibiotic sensitivity, and glucose utilizationwere analyzed for four isolates, and finally SRCM 100731 wasselected. SRCM 100731 was named as Bacillus amyloliquefaciensSRCM 100731 16S rRNA sequencing analysis, and carried outoptimization of cell growth for industrial applications such aspet food and feed. The effects of 14 different components oncell growth were investigated and three significant positivefactors, molasses, sodium chloride, and potassium chloridewere selected as the main factors based on a Plackett-Burmandesign. In order to find out optimal concentration on each constituent,we carried out central composite design. The predictedoptimized concentrations were 7% molasses, 1.1% sodiumchloride, 0.5% potassium chloride. Finally, an overall about7-fold increase in dry cell weight yield (12.6625 ± 0.0658 g/L)was achieved using the optimized medium compared with thenon-optimized medium (1.8273 ± 0.0214 g/L). This research isexpected to be highly utilized in the growing pet industry byestablishing optimal cultivation conditions for industrial applicationas well as screening Bacillus amyloliquefaciens SRCM100731 as probiotic resource for companion animal.
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KEYWORD
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Bacillus amyloliquefaciens, companion animal, isolation, optimization, probiotics
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